Hamilton Microlab STAR - Page 72

Microlab
®
STAR User Manual
Page 72 of 225 610766/02
Standard or aliquoting mode must be selected. For VWDQGDUGSLSHWWLQJ mode, three different
procedures are possible:
- “Simple“ pipetting for simple aspiration/dispense cycles,
- “Replica“ for cycles of aspirations/dispenses where the liquid from one source is
dispensed into multiple target containers (no aliquoting), e.g., for a “double test“
(tube 1 -> plate position A1, A2, tube 2 -> plate position A3,A4, etc.). The desired multiple
can be chosen (e.g. 2 for a double test).
- “Pooling“ for cycles of multiple aspirations/dispenses where liquid from multiple source
containers is dispensed into one target container (e.g., tube 1, tube 2 -> plate position
A1, tube 3, tube 4 -> plate position A2, etc). Here too, the desired multiple has to be
chosen (e.g., 12 for pools of 12).
For the aliquoting procedure, the choices under pipetting mode are deactivated. Aliquoting
means that one aspiration is followed by multiple dispenses (e.g., tube 1-> plate positions A1
to A12).
The next step is to select the appropriate tip handling:
First, select the tip sequence - where the tips are to be picked up from. The tip type is
automatically retrieved from the sequence. If the tip sequence is used up, it will be
automatically reloaded. If “Reducible by user“ is checked, the tip sequence can be reduced
on reloading.
7LSKDQGOLQJ can be chosen to
- replace tips “after each dispense“ (if multiple dispenses are needed to transfer e.g. 900 µl
with a standard tip of 300 µl),