Table of Contents EPC9 Manual 3
5. E9SCREEN Software 35
EPC9 Window _________________________________________________ 35
Main Controls.................................................................................................................... 35
Hidden Controls................................................................................................................. 46
Notebook Window_______________________________________________ 48
Drop-Down Menus ______________________________________________ 48
File Menu ......................................................................................................................... 48
Edit Menu......................................................................................................................... 49
EPC9 Menu ...................................................................................................................... 50
Notebook Menu ................................................................................................................. 52
Calibrate Menu .................................................................................................................. 53
6. Operating Modes 54
Voltage-Clamp Mode _____________________________________________ 54
Current-Clamp Mode _____________________________________________ 54
Test Mode ____________________________________________________ 57
Search Mode __________________________________________________ 58
7. Compensation Procedures 59
Series Resistance Compensation ____________________________________ 59
Capacitance Compensation ________________________________________ 62
Offset Compensation_____________________________________________ 63
8. Patch-Clamp Setup 67
Mounting the Probe ............................................................................................................ 67
Ground Wires.................................................................................................................... 67
Grounding the Microscope ................................................................................................... 67
External Shielding .............................................................................................................. 68
Connections to other Instruments .......................................................................................... 68
Pipette Holder and Electrode ................................................................................................ 68
Bath Electrode................................................................................................................... 69
9. Patch-Pipettes 71
Glass Capillaries................................................................................................................ 71
Pulling.............................................................................................................................. 72