E9Screen Software EPC9 Manual 47
Sound Settings: Sensitivity (Hz/MΩ) and volume
(in %) of the sound encoding of R-membrane can be
specified here. To enable the sound option press the
Sound button.
CC Fast Speed: Clicking on this button enables the fast current-
clamp mode (only version “C” and later versions of the EPC9).
For details see Chapter 6. Operating Modes on page 54).
Gentle CC-Switch: This control selects between the
two alternate modes of switching between voltage
and current clamp and back.
When selecting Gentle CC-Switch: On, I-hold will be set to inject the holding current
required to keep the membrane voltage constant while switching to current clamp
mode. Thus, V-mon will be identical to the original V-membrane commanded under
voltage clamp. In analogy, upon returning to voltage clamp, V-membrane will be set
according to the membrane potential under current clamp and rounded to the next 2
mV.
When selecting Gentle CC-Switch: Off, I-hold will be set to 0 pA when switching to
current clamp mode.
Last V-membrane: Quite often, the membrane potential under
current clamp will have changed, and when returning to voltage
clamp, V-membrane will possibly differ from that set before switching to current-
clamp mode. Last V-membrane can then be used to conveniently restore the original
V-membrane value.
CC-range: Two current clamp scaling ranges can be
selected: 1 and 10 pA/mV (with the exception of the
EPC9 version “A” and “C”, which have a fixed CC-scaling of 1 pA/mV). When the
CC-scaling is set to 1 pA/mV, a command pulse of 1 mV gives rise to 1 pA inCurrent
Clamp mode. This relationship of 1 and 10 pA/mV holds regardless of the Gain
setting. The maximal output current inCurrent Clamp mode is 1 and 10 nA,
respectively.
Relative Value: This button is useful when recording
macros. A change of any value will be recorded as a
relative change, e.g. if you hyperpolarize the holding potential from -60 to -70 mV
while recording a macro, a step of -10 mV will be recorded (see description of Macros
at the beginning of the subchapter Main Controls).
Sound
Volume
Hz/MΩ Rmem
100 %
100
Gentle CC-Switch: ON
CC-range: 1pA/mV
Relative Value
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