Patch-Clamp Setup EPC9 Manual 70
Ag-AgCl wire
agar
The body of the electrode is a 1 ml plastic syringe body that has been heated and
pulled to form a small, bent tip. The electrode proper is a chlorided Ag wire that is
first inserted with the plunger into the fluid-filled body; then hot agar is sucked into
the tip by withdrawing the plunger partially. The filling solution can be a typical
bath solution or something similar, such as 150 mM NaCl. More concentrated salt
solutions are not necessary, and they can leak out, changing the composition of the
bath solution.
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