Clumped cells
Gently but thoroughly pipette your cell suspension to break up aggregates prior to
counting.
Too few or too many cells
Cell concentrations of 5 x 10
5
-5 x 10
8
cells/mL are optimal for counting.
Dilute or concentrate cell suspensions accordingly.
Fluorescence signal too strong or weak
Adjust light intensity.
Visible cells uncounted
Adjust the protocol’s detection sensitivity.
Improper slide insertion
Make sure that the slide has been pushed completely into the end of the slide port.
Improper sample loading
Do not over- or under-fill the slide chambers.
Slowly load the chambers with 5-6 µL of cell suspension (10-30 seconds).
Optical components malfunctioning
Optical components may be dirty or damaged.
Please contact your local distributor or Logos Biosystems.
Damaged or contaminated slide
Use a new QUANTOM
™
M50 Cell Counting Slide.
Wear gloves and handle by the edges to avoid smudging and contamination.
Incorrect dilution factor
Adjust the dilution factor in the selected protocol or create a new protocol.
Make sure the appropriate dilution factor has been selected.
Poorly aligned images
Centrifuge cell suspension sample appropriately.
Incompatible USB drive
Some USB devices are undetectable or incompatible. Use the USB supplied with the
instrument or use a USB 2.0.
Failed wireless connection
Make sure the WiFi dongle is connected to the QUANTOM Tx
™
. Make sure the
QUANTOM Tx
™
is connected to a wireless network. Make sure the PC is connected to the
same wireless network as the QUANTOM Tx
™
. Check your wireless network connection.
Freezing during background calibration
If calibration takes more than 10 minutes, reset the system by turning the power off and
then on. If calibration fails repeatedly, contact your local distributor or Logos Biosystems.
More than one software version on the USB drive
Delete previous versions of software from the USB drive before downloading new
software.
Incompatible USB drive
Some USB devices are undetectable or incompatible. Use the USB drive supplied with the
instrument or use a USB 2.0.
Incorrectly saved or damaged software
Download the file again into the root directory of the USB drive. Insert the USB drive and
press Software Updates in the Settings screen. If the problem persists, contact your local
distributor or Logos Biosystems.
To Next Page
Loading...