2
Table of Contents
Safety Precautions .................................................................................................................................................... 4
1. Product Introduction ............................................................................................................................................. 5
Product Contents .............................................................................................................................................................. 5
Product Description .......................................................................................................................................................... 5
QUANTOM Tx
™
Microbial Cell Counter ........................................................................................................................... 5
QUANTOM
™
Centrifuge ................................................................................................................................................. 6
QUANTOM
™
M50 Cell Counting Slides ........................................................................................................................... 6
2. Getting Started ...................................................................................................................................................... 7
Installation ....................................................................................................................................................................... 7
Setup ............................................................................................................................................................................... 7
QUANTOM
™
Centrifuge settings .................................................................................................................................... 7
QUANTOM Tx
™
startup ................................................................................................................................................. 7
3. Counting Cells ....................................................................................................................................................... 8
Sample Preparation .......................................................................................................................................................... 8
Sample staining ........................................................................................................................................................... 8
Sample loading ............................................................................................................................................................ 8
Slide centrifugation ...................................................................................................................................................... 8
Counting with the QUANTOM Tx
™
..................................................................................................................................... 8
Slide insertion/removal ................................................................................................................................................. 8
Viewing images ............................................................................................................................................................ 8
Cell counting ................................................................................................................................................................ 9
Next steps ................................................................................................................................................................... 9
Results ........................................................................................................................................................................... 10
Image view................................................................................................................................................................ 10
Histograms ................................................................................................................................................................ 10
Dilution calculator ...................................................................................................................................................... 11
Save .......................................................................................................................................................................... 11
Print .......................................................................................................................................................................... 12
Quick save ................................................................................................................................................................. 12
4. Reviewing/Exporting Data .................................................................................................................................. 13
Review/Export Data on the QUANTOM Tx
™
...................................................................................................................... 13
Review images ........................................................................................................................................................... 13
Previous counts ......................................................................................................................................................... 13
Reanalyze .................................................................................................................................................................. 13
WiFi Data Transfer .......................................................................................................................................................... 14
QUANTOM
™
Utility ...................................................................................................................................................... 14
FTP freeware ............................................................................................................................................................. 15
5. Protocols .............................................................................................................................................................. 16
Protocol Parameters ....................................................................................................................................................... 16
Counting parameters .................................................................................................................................................. 16
Dilution factor ............................................................................................................................................................ 16
Fluorescent object size ............................................................................................................................................... 16