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Autoclaving
AUTOCLAVING
1. Remove the piston assembly from the cylinder.
2. Remove and discard the cross tubes into a biohazardous
container.
3. Remove the o-rings (only if necessary, see section “Replacing
O-rings”) and discard them into a biohazardous container.
4. Place all components (see Figure 5) into the autoclave and
autoclave at 121 °C for 20 minutes.
Figure 5: Components to be Autoclaved
5. After autoclaving, place all parts on a paper towel. The cylinder
should be inverted to allow for run-off of excess water. Allow
the parts to dry for approximately one hour.