3-4
  Endpoint 
1  Prepare the samples and calibrators and controls. 
Prepare proper amount of reagent according the number of the samples. 
If it is double-reagent analysis, it is recommended that R1 and R2 are 
mixed and then dispensed into respective tubes (or other reaction 
containers). 
 
2 
 
NOTE 
Check the reagent instruction, or consult the 
reagent manufacturer or distributor to see if mixing 
of R1 and R2 are allowed prior to testing. 
 
3  Dispense sample or calibrator or control into the tube. 
4  Incubate the tube in a water bath tank or external incubator for a while. 
5  Take the tube out to prepare it for analysis. 
6 Click Test on the main screen to enter the test screen. 
7  Select the name of the test to be run. 
8 Click Water Blank, put the inlet tube into the distilled water, and click 
Aspirate. 
9 Click Reagent Blank, put the inlet tube into the reagent blank, and click 
Aspirate. 
10 Select Calibrate and select calibrator in the drop down list box of S1-S8. 
Put the inlet tube into the calibrator to be tested and click Aspirate. If it is 
multiple-point calibration, you can proceed to the next step only when the 
calibration tests of all the calibrators are completed. Put the inlet tube 
into the sample blank, and click Aspirate. 
12 
 
NOTE 
You can determine whether it is necessary to run 
sample blank.   
Hemolysis, icterus or lipemia in the samples may 
lead to unreliable test results, so a sample blank is 
recommended. 
 
13  Select Sample ID and click Sample. Put the inlet tube into the sample 
and click Aspirate. Repeat this to test other samples. 
14  After calibration is finished. Click QC and select controls from the 
drop-down list box C1-C3.  Put the inlet tube into the control to be 
tested and click Aspirate. 
15  After all the tests are completed, put the inlet tube into the distilled and 
click Wash. 
  Fixed-time and Kinetics 
1  Prepare the samples, calibrators and controls. 
Prepare proper amount of reagent according the number of the samples. If it is 
double-reagent analysis, it is recommended that R1 and R2 are mixed and 
then dispensed into respective tubes (or other reaction containers).