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IV. Microscopic Procedure
1. Interpupillary Distance Adjustment
y Before adjusting the interpupillary distance, bring a specimen into focus using the 10x objective.
y Adjust the interpupillary distance so that both the right and left field of view become one. This
adjustment will enable the user to observe the specimen with both eyes.
2. Diopter Adjustment
y Diopter adjustment compensates for the differences in vision between the left and right eyes. In
addition to making observation through both eyes easier, this adjustment also reduces the extent to
which focusing is lost when the objective magnification is changed. In particular, this occurs when a
low magnification objective is used.
y Before adjusting the diopter, bring a specimen into focus using the 10x objective.
y Turn the diopter compensation ring on each eyepiece until the adjustment ring is adjusted to the“0”
position.
y
(Fig.18) (Fig.19)
y Position the 40x objective into the optical path and bring the specimen image into focus by turning
the coarse and fine focus knobs.
y Position either the 4x or 10x objective into the optical path. Without adjusting the fine and coarse
focus knobs, turn the diopter rings on the eyepieces so that the specimen images in the left and
right eyepieces are focused individually.
y Repeat the above step twice.
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