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VI. Troubleshooting Table
As you use your microscope, you may occasionally experience a problem. The troubleshooting table
below contains the most frequently encountered problems and their possible causes.
1. Optical and Operating Problems
Problem Possible Cause
Vignetting or uneven brightness in the field
of view or field of view only partially visible
Bulb not installed properly
Filter slider in intermediate position
Phase slider not in click-stop position
Incorrect condenser mounting
Aperture diaphragm closed too far
Revolving nosepiece not clicked into position
Optical path selector lever in intermediate position
(Mod. AE2000 trinocular only)
Dust or dirt in field of view
Aperture diaphragm closed too far
Dust or dirt on specimen’s surface
Image quality:
No image under phase contrast or details
cannot be viewed
Brightfield objective being used
Phase annular diaphragm not in optical path
Phase annular diaphragm and objective phase symbol
do not match
Slider annular ring image has moved away from the
objective phase plate image
Thickness of specimen holder is outside the
compensating range of objective
Eye strain or fatigue
Interpupillary distance not adjusted
Diopter adjustment not made
Inadequate illumination
Field of view of left and right eyepiece differ
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