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5. PHOTOMICROGRAPHIC PROCEDURE
To ensure vibration free operation, set the microscope on a sturdy vibration free table or a bench
with a vibration proof device.
Pull the optical path selection lever of the trinocular eyepiece tube all of the way out to the limit, the
ratio of light entering the observation tube and phototube will be 0:100.
For the same total magnification, select a combination of the highest possible objective
magnification and lowest possible projection lens magnification to achieve the utmost
imagedefinition and contrast.
To ensure optimal illumination, check the position and centring of the lamp and position of the
condenser.
Select a blue filter for routine application. An additional colour-compensating filter can also be used
depending on the colour rendition.
Adjusting the field diaphragm is important for the purpose of limiting extraneous light that may
cause flare and lower the contrast. Stop down the diaphragm to achieve an illuminated area slightly
larger than that of the field of view.
A change of depth of focus, contrast and resolution of image is attainable with an aperture setting
that is 2/3 of the objective N.A.
For specific photomicrographic procedures, refer to the manual of the specific camera being used.
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