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Nikon Eclipse 50i POL - Eyepiece Tube

Nikon Eclipse 50i POL
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Chapter 2 Microscopy
2.1 Diascopic Bright-field Microscopy
17
5
Move the Bertrand lens turret to
the “O” position to remove the
Bertrand lens from the optical
path.
P.48
6
Move the 10X objective into the
optical path.
7
Set the specimen in place with the
cover glass facing up.
8
Raise the condenser as high as it
will go.
9
Fully open the field diaphra
g
m and
the condenser aperture
diaphragm.
10
Set the optical path to make
100% light go into the binocular
part when using the trinocular
eyepiece tube.
P.33
Remove the
Bertrand lens
from the optical
p
ath.
Set the optical path to
make 100% light go into
the binocular part.
Set the 10X
objective.
Set the
specimen with
the specimen
holders.
Raise the condenser
with the condenser
focus knob.
Fully open the
aperture
diaphragm with
the aperture
diaphragm
knob.
Fully open the field
diapahram with the field
diaphragm control.

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