Chapter 3 Individual Operations
3.15 Water Immersion Operation
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3.15
Water Immersion Operation
Objectives marked “WI” or “W” are water-immersion type objectives. These objectives are used
with immersion water (distilled water or physiological saline) applied between the specimen and
the tip of the objective. Microscopy procedures are the same as for oil-immersion type
objectives.
Since water evaporates readily, monitor the immersion water during observation. Avoid using
too much water, since excess water will flow onto the stage and around the condenser,
promoting corrosion.
Wipe off water
After use, wipe off water from the tip of the objective and condenser, then follow up by wiping
with absolute alcohol.
If you observe water stains, apply a small amount of neutral detergent and wipe gently, then
follow up with absolute alcohol.
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