Chapter 3 Individual Operations
3.18 Image Capturing
54
3.18
Image Capturing
Microscopy images can be captured by attaching a camera head to the trinocular eyepiece tube.
For detailed information, refer to the operating manual provided with the camera head or
camera control software.
Proper adjustment of light intensity and focus on the microscope are important for obtaining
clear images. Listed below are key considerations in capturing clear images.
3.18.1
Adjusting Light Intensity
• Lamp voltage: If accurate color reproduction is critical, set the brightness control
knob to a midpoint setting and use the ND filters to make brightness
adjustments.
• Filter: Place a commercially available color compensation filter on the filter
pocket at the microscope base, as necessary.
3.18.2
Adjusting the Condenser
• Focus and center the condenser always.
• Center the annular diaphragm for the phase contrast microscopy.
• For normal operations, set the diaphragm aperture to 70 to 80% of the N.A. of the
objective.
3.18.3
Confirming the Photomicrographic Range
The image on the monitor represents the photomicrographic range.
3.18.4
Confirming the Focus
Check the focus by viewing through the eyepiece and viewing the monitor. If the focal positions
for the two images differ, adjust the focal position adjustment screw at the camera port.
To Next Page
Loading...
