Chapter 3 Individual Operations
3.17 Episcopic Microscopy
51
3.17
Episcopic Microscopy
To perform episcopic microscopy, attach the LV-UEPI universal epi illuminator to the microscope.
3.17.1
Switching the Episcopic Illumination
You can switch the illumination between the
bright-field and the dark-field to be used for the
episcopic microscopy by operating the illumination
selection lever on the right side of the epi illuminator.
For the bright-field, push in the lever.
When the lever is pulled out, the dark-field is specified.
But you cannot perform the episcopic dark-field
microscopy using this microscope.
* The dark-field setting (DF) is used for the diascopic
illumination.
Lever position Illumination method
Push in (BF) Episcopic bright-field ( and Episcopic polarization microscopy)
Pull out (DF) Diascopic illumination (The dark-field microscopy is not available.)
3.17.2
Field Diaphragm in the Epi Illuminator
The field diaphragm restricts illumination to the area of
the specimen that is being viewed. Operating the field
diaphragm open/close lever changes the size of the
field diaphragm. For normal observation, the size of
the diaphragm should be such that it is just outside (or
inside) the edge of the viewfield. If a broader area than
necessary is illuminated, stray light will enter the
optical system, creating flaring, and reducing the
contrast of the optical image.
Appropriate field diaphragm settings are particularly
important for photomicrography and digital image
capturing. In general, good results will be obtained by
stopping down the field diaphragm to settings slightly
wider than the area to be reproduced within the photo
frame or monitor display.
Field diaphragm
centering screw
(both side)
Field diaphragm
open/close lever
Illumination selection lever
Field diaphragm
ima
e
Eye piece viewfield
To Next Page
Loading...
