Chapter 3 Individual Operations
3.6 Diopter Adjustment
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3.6
Diopter Adjustment
Diopter adjustment compensates for differences in visual acuity between the right and left eyes,
improving binocular observation. It also minimizes focal deviations when switching objectives.
In the case of a polarizing microscope, since an eyepiece containing crosshairs is used for the
right eye, the procedure for adjusting the diopter differs from that of an ordinary microscope.
(1) Observe the right eyepiece with the right eye.
Turn the diopter adjustment ring to bring the
crosshair in the eyepiece into focus.
(2) Still observe the right eyepiece with the right
eye. Turn the fine/coarse focus adjustment
knob to bring the specimen on the stage into
focus.
(3) Observe the left eyepiece with the left eye.
Turn the diopter adjustment ring on the
eyepiece to bring the specimen into focus.
(not the fine/coarse focus adjustment knob)
3.7
Interpupillary Distance Adjustment
Interpupillary adjustment improves the ease of
binocular observation.
Perform steps 1 to 12 in “2.1 Diascopic Bright-Field
Microscopy” and focus on the specimen using the
10x objective. Then, move the eyepiece sleeve until
the view fields for the right and left eyes coincide.
View field
Converge until
the right and left
view fields
coincide.
(2) Focus on the specimen with the
right eye.
(3) Focus on the
specimen with
the left eye.
(1) Focus on the
crosshair with the
right eye.
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