Chapter 2 Microscopy
2.3 Conoscopic Observation
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2.3
Conoscopic Observation
This section describes the conoscopic observation procedure. This is the characteristic
observation method of polarizing microscopes. In this method, the specimen is observed with
the polarizer, the analyzer, and the Bertrand lens placed in the optical path.
The specimen can be observed from various angles with diascopic light in the form of a single
image. However, the shape of the specimen itself is not visible with this observation. You can
distinguish the property of the specimen between uniaxial and biaxial and observe the optical
axial angle and optical characteristics of the specimen.
1
Perform the orthoscopic observation. (Refer to “2.2
Orthoscopic observation.”)
2
Rotate the Bertrand lens turret to
the “B” position to move the
Bertrand lens into the optical
path.
3
Focus and center the Bertrand
lens.
⇒ P.48
4
Perform the conoscopic microscopy.
• The P-CL 1/4 λ & tint plate is not used in this microscopy. Move it to the vacant position.
• Select an objective having a large numerical aperture (high magnification: normally 40X or
higher)·
• The condenser aperture diaphragm should be adjusted so that its image circumscribes the
conoscopic view field or should be fully opened.
• The field diaphragm should be adjusted so that its image circumscribes the conoscopic view
field.
• The top lens of the swing-out condenser must be placed in the optical path.
Move the
Bertrand lens
into the optical
path.
Center the Bertrand
lens using the two
screws.
Focus the
Bertrand lens.
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