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ECLIPSE Ni-E
Nikon ECLIPSE Ni-E User Manual
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Chapter 2
Microscopy
Oper
ations
32
Bright/Dark-field
Microscopy
Preparation
Focus and Optical
System A
d
justment
Micro-
scopy
Bright/Dark-field
Microscopy
1
2
3
4
5
6
7
8
9
10
11
12
13
14 15 16 17 18
19
19
Turn off the power.
T
urn of
f the power switches (press to the
“O” position) on
the control box
A
and connected motorized
devices.
(Each power LED turns of
f.)
50
60
70
T
urning off the device
Power switch
OFF
Power LED OFF
Power LED OFF
Power LED OFF
Power switch
OFF
Power LED OFF
45
47
Table of Contents
Default Chapter
3
Introduction
3
Contents of the Manual
4
Symbols Used in this Manual
4
Differential Interference Contrast
5
Summary of Contents
5
Table of Contents
6
Safety Precautions
8
WARNING and CAUTION Symbols
8
Meaning of Symbols Used on the Product
8
Warning
8
Caution
8
Notes on Handling the Product
13
Components
15
System Configuration and Controls for Bright/Dark-Field Microscopy
16
Components
16
Controls
17
Buttons
18
Connectors
19
System Configuration and Controls for Epi-Fluorescence Microscopy
20
System Configuration and Controls for Differential Interference Contrast Microscopy
22
System Configuration and Controls for Phase Contrast Microscopy
23
Microscopy Operations
25
Before Microscopy
26
Operation Flowchart
28
Bright/Dark-Field Microscopy Procedure
32
Lower the Condenser Slightly from the Uppermost Position
34
Fully Open the Field Diaphragm and Aperture Diaphragm
35
Focus on the Sample
38
Adjust the Interpupillary Distance
39
Focus and Center the Condenser
39
Oil Immersion
41
Adjust the Aperture Diaphragm
42
Adjust the Field Diaphragm
43
Epi-Fluorescence Microscopy Procedure
47
Differential Interference Contrast Microscopy Procedure
63
Phase Contrast Microscopy Procedure
81
Individual Operations
97
Display Panel Details
98
Using Operation Buttons on Ni-E
101
Front Operation Button
101
Right Operation Button
102
Left Operation Button
103
Adjusting the Brightness of a Diascopic Image
104
Adjustment by Lamp Voltage
104
Adjustment with ND Filters
105
Focusing on the Sample (Vertical Stage Movement)
106
Proper Focusing Procedure
106
Focus Knobs on the Main Body
107
Resetting the Z-Axis Coordinate
108
Refocusing
109
Bringing the Target into the Optical Path (Horizontal Stage Movement, Rotation)
110
Operating the Rotatable Ceramic Coated Stage
110
Operating the Motorized XY Stage
112
Adjusting the Diopter
113
Focusing and Centering the Condenser
114
Adjusting the Aperture Diaphragm
115
Using the Condenser
117
Compatibility of Condensers with the Magnification of Objectives
117
Using the NI-CUD-E Motorized Universal Condenser (Dry)
118
Using the NI-CUD Universal Condenser (Dry)
119
Using the D-CUO DIC Condenser (Oil)
121
Adjusting the Field Diaphragm
122
Switching the Objective
123
Motorized Nosepiece Operation
123
Intelligent/Manual Nosepiece Operation
125
Switching the Optical Path of the Tube
126
Optical Path Switching of the Motorized Quadrocular Tilting Tube
126
Switching the Optical Path in a Manually-Operated Tube
127
Adjusting the Binocular Section
128
Adjusting with the Binocular Section of the Tube
128
Using the Eyelevel Riser
128
Oil/Water Immersion
129
Dark-Field Microscopy
131
Epi-Fluorescence Microscopy
132
Epi-Fluorescence Cube Turret Operation (Switching Excitation Methods)
132
Selecting Filters
134
Protecting the Sample and Preventing It from Decoloration (Using the Shutter)
136
Adjusting the Brightness of the Fluorescent Image (Using ND Filters and the Aperture Diaphragm)
138
Restricting the Illumination to the Area of the Specimen Being Viewed (Centering and Adjusting the Field Diaphragm)
140
Changing the Waveform Characteristics of the Excitation Light (D-FB Excitation Balancer)
141
Switching the Excitation Filter (Motorized Excitation Filter Wheel)
142
Switching the Barrier Filter (Motorized Barrier Filter Wheel)
143
Other Notes on Epi-Fluorescence Microscopy
143
Differential Interference Contrast Microscopy
144
Tips for Differential Interference Contrast Microscopy
144
Using Optical Elements
145
Phase Contrast Microscopy
146
Tips for Phase Contrast Microscopy
146
Using Optical Elements
147
Useful Functions
148
Mode
148
Interlocking Function
152
Using the Ergo Controller
155
Using the Joystick Controller
158
Capturing Images
159
Operation on DS-L3
164
Setting up the Microscope
164
Controlling the Microscope
180
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Nikon ECLIPSE Ni-E Specifications
General
Brand
Nikon
Model
ECLIPSE Ni-E
Category
Microscope
Language
English
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