Chapter 2 Microscopy Operations
42
Epi-fluorescence
Microscopy
Preparation Focusing Microscopy
Epi-fluorescence
Microscopy Procedure
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Switch from Epi-Fluorescence Microscopy to Bright-field Microscopy
(1) When switching from epi-fluorescence to bright-field
microscopy, close the epi-fluorescence illumination path
shutter and block the illumination of the mercury lamp.
The shutter is closed when the [FL SHUTTER] button is
lit.
ND
8
ND
32
OUT
IN
NCB
11
Closing shutter
(2) Rotate the epi-fluorescence cube turret to bring turret
address 1 into the optical path. (If a filter cube is in
address 1, remove it in advance.)
ND
8
ND
32
OUT
IN
NCB
11
Removing the filter cube
PF__2x____Z:_____0.000um
OPEN
'
C _____ND_ 1
'
C __EPI:C
Empty position Shutter closed
(3) Press the dia-illumination ON/OFF switch to turn on the
lamp.
Dia-illumination lamp ON
(4) Focus and center the condenser. (See Chapter 3 “7
Focusing and Centering the Condenser”.)
For details on other operations and precautions, see “3
Bright/Dark-field Microscopy Procedure”.
Lit
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