Multi Area Time Lapse
（with the Z drift compensation）
*Before starting the following procedure,
make adjustments for XY imaging.
ZDC Setting (See page 10 for details. )
① In [▼Z Limit Setting], press the
button to set the
Near limit to all objective lenses.
② In [▼Z Section] in [Series] Tool Window,
press the to set current position 0.
⑤ Set [ZDC DM] in [▼ZDC Control] in
[Microscope] Tool Window to “In” and
place the dichroic mirror of ZDC in the
light path.
⑥ Specify the search zone of the coverslip
position in [Upper Limit] / [Lower Limit]
in [Search Zone] using the Origin
coordinate of Z as a base point.
⑦ Set [Z Drift Compensation in series scan]
in [ZDC Control] in [Microscope] Tool
Window to "ON".
⑧ Adjust focus and Press the
button of [Coverslip position]. The
coverslip top surface position is acquired.
When the Z drift compensation is
successful, the buzzer sounds beeps
once only. If the Z drift compensation is
not successful, the buzzer sound beeps
three times.
⑨ Press the button to register areas.
⑩ set [Cycle interval] and [Cycle].
Starting Multi Area Time Lapse
⑪ Select [MATL] tab in [Acquire] Tool
Window.
⑫ Press the button to display the dialog
box, and select the folder to save the
images.
⑬ Press button to start acquiring the
image.
◆ZDC◆
11
⑦
⑩
⑪
⑫
⑬
◆motorized stage◆
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