Olympus FV3000 - Processing: Unmixing（３

Processing: Unmixing（3）
Normal Unmixing
〜by specifying dye dates
① Press the button to switch
to “Viewer mode”.
② Select [Analysis] in [Tool Window]
menu. [Analysis] Tool Window appears.
③ Select [Single process] mode, press the
[Post Processing] button.
④ Press the button to reset the
assigned item and select [Spectral
Deconvolution] in [Processing Item].
⑤ In [Input] in [Input / Output setting],
select the image for image processing.
⑥ In [Mode] in [processing Property], select
“Normal Unmixing”.
⑦ In “!DYE0 Dye Profile Load File”, select
the first dye profile among from dye
data. (See page 30. )
⑧ Select the all dye data.
⑨ Press the button to start the
fluorescent separation process.
③
②
④
⑤
⑥
⑦
⑧
⑥
④
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