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Olympus FV3000 User Manual

Olympus FV3000
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XY Image Acquisition(1)
Select “VBF” in the [PMT Setting] Tool Window.
Assigning the detector to channel
Press the button on [PMT Setting]
Tool Window to open the [Dye & Detector Select]
dialog box.
Press the button to reset the Assigned
Dye.
Double-click the name of the fluorescence dye to
observe.
When registering a fluorescence dye, TD channel
is registered automatically. If you don’t need,
double-click the TD channel to cancel it.
If you want to observe with multiple phases using
the virtual channel scan, press the button
to add the phase. And drag&drop the selected
“Dye” to the observation channel list of the phase
you want to add.
After setting all channels, press the [OK] button.
Adjusting the live image
If you want to acquire multiple channel image,
select Sequential scan “Line” in the [PMT Setting]
Tool Window.
Press the button on the [Live] window.
Adjust focus and set Laser Intensity(%),
Sensitivity(HV), Gain and Offset on [PMT setting]
Tool Window.
If you use virtual channel scan, press
to switch phases. On both phases, adjust focus
and set Laser Intensity(%), Sensitivity(HV), Gain
and Offset.
Virtual Channel Scan
This mode allows to acquire multiple CH image
whose channel number is larger than the
number of detector.
Adjusting Confocal
Aperture.
Reducing noises by
Averaging.
Laser Intensity
Sensitivity
4
【Hi-Lo Mode】
pixels of intensity 4095 are shown in red.
Pixels of intensity 0 are shown in red.
u5

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Olympus FV3000 Specifications

General IconGeneral
BrandOlympus
ModelFV3000
CategoryMicroscope
LanguageEnglish

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