© 2024 PacBio. All rights reserved. For research use only. Not for use in diagnostic procedures.
PN 103-140-400 REV05 JUN2024
At the top of the cluster generator, open the workspace door by lifting the cover upwards.
Unload previous run consumables:
• Remove the wash cartridges if loaded, by gently pinching the sides of the wash cartridges and lifting up
and out of the instrument slots.
• Place the wash cartridges in the wash cartridge storage locations in the instrument reagent drawer.
Wash cartridges are safe to use for up to 30 days from initial use.
• If a previous clustering reagent plate is found loaded in the instrument reagent drawer, dispose of the
previous clustering reagent plate and its contents in the appropriate waste stream.
• If a clustering instrument wash plate and troughs are found loaded in the instrument reagent drawer,
remove and clean the troughs by rinsing with DI water and drying with a lint-free wipe. Store the plate and
troughs upside down or in a bag to prevent dust accumulation between uses.
Lift the flow cell clamp upwards to allow for loading of a new flow cell.
Open the Onso flow cell kit:
1. Hold the base of the flow cell kit in one hand with the PacBio logo forward-facing. Lift and roll the top-
front of the box towards the back.
2. Lift the lid of the flow cell kit upward and set aside.
Load the flow cell kit onto the cluster generator, by placing the bottom base of the opened flow cell kit onto
the cluster generator workspace area, such that the PacBio logo on the base of the flow cell kit is facing the
user and the front of the flow cell kit base is flush with the front of the workspace area. This will cause the
base of the flow cell kit to sit against the flow cell clamp hinge.
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