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PN 103-140-400 REV05 JUN2024
Remove the flow cell kit base from the instrument workspace. Retrieve the flow cell kit lid. Close the flow cell
kit lid:
1. Hold the base of the flow cell kit in one hand with the PacBio logo forward-facing. Seat the back of the
flow cell kit lid onto the base.
2. Roll the top-front of the flow cell kit lid towards the PacBio logo.
3. Press down to secure, being careful not to pinch any fluidics tubing between the kit lid and base.
Retain the clustered flow cell in the flow cell kit– this flow cell is ready for sequencing on the sequencer.
If not sequenced immediately, it can be stored at 4°C for up to 1 week.
Select “Open workspaces” on the software screen. Retrieve the wash cartridges from within the cluster
generator drawer and align one on each side with the instrument connector slots in the cluster generator
workspace. Press down to engage wash cartridges until an audible click is heard.
It is encouraged to verify wash cartridge engagement by gently pulling the wash cartridges
upward. If properly installed, the wash cartridges should not easily disconnect from the cluster
generator.
Lower the magnetic flow cell clamp and close the workspace door.
Prepare at least 30 mL of fresh 0.1% hypochlorite (bleach) solution.
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