© 2024 PacBio. All rights reserved. For research use only. Not for use in diagnostic procedures.
PN 103-140-400 REV05 JUN2024
• If wash cartridges are found loaded, remove them by gently pinching the sides of the wash cartridges and
lifting up and out of the instrument slots. Place the wash cartridges in a plastic bag for future use.
Wash cartridges are safe to use for up to 30 days from initial use.
• If a previous sequencing reagent pack is found loaded in the instrument reagent door, dispose of the
previous sequencing reagent pack and its contents in the appropriate waste stream. Refer to the section
titled “Dekitting and Reagent Discard” for detailed instructions.
• If a sequencing instrument wash pack is found loaded in the instrument reagent door, remove and clean
the wash pack by rinsing with DI water and drying by inverting and tapping gently upside down. Dry the
exterior of the wash pack with a lint-free wipe. Store the wash pack upside down or in a bag to prevent
dust accumulation between uses.
Empty the internal waste container and return the container to the instrument. Ensure that the waste
container is loaded correctly under the waste line spout. See section “Dekitting and Reagent Discard” for
detailed instructions.
Open the flow cell kit containing the clustered flow cell:
1. Hold the base of the flow cell kit in one hand with the PacBio logo forward-facing. Lift and roll the top-
front of the box towards the back.
2. Lift the lid of the flow cell kit upward and set aside.
Load the flow cell kit containing the clustered flow cell onto the sequencer, by placing the bottom base of the
opened flow cell kit onto the sequencing instrument workspace area, such that the PacBio logo on the base of
the flow cell kit is facing the user. The cutout in the flow cell kit base should wrap around the preload cover.
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