Load the flow cell in the sequencer instrument workspace:
1. Lift the flow cell out of the kit base and wipe clean with isopropyl alcohol:
a. Holding either the left or right wing of the flow cell, lift the flow cell directly upward out of the
kit base. In this orientation, the bottom of the flow cell is facing the user.
b. Wipe clean the front and back of the flow cell surface using a lint-free wipe saturated with
70% isopropyl alcohol.
c. Allow the 70% isopropyl alcohol to dry from the flow cell surface or wipe dry with a new lint-
free wipe.
2. Mount the flow cell onto the instrument metal guide pins:
a. Rotate the flow cell away from the user and over the preload cover. The QR code must be in
the upper right-hand corner, and the top of the flow cell facing the user once rotated.
b. Align the pinholes on the flow cell with the metal guide pins on the sequencer in the
workspace area. The flow cell wing with two pinholes (circle and diamond) will be positioned
on the left wing and the wing containing one pinhole (oval) will be positioned on the right.
c. Slide the flow cell onto the guide pins, away from the user.
3. Engage the left and right MICA:
a. Remove the left and right MICA from the flow cell kit base and align the MICA instrument
connector sides with the instrument connection slots such that the fluidic tubing is towards
the center of the instrument, while the QR code is pointed towards the left and right sides of
the instrument, respectively.
b. Connect both left and right MICA by gently pushing each down into their respective slots
until an audible click is heard for each. The audible click indicates that the MICA has been
engaged.
It is encouraged to verify MICA engagement by gently pulling the MICA instrument connectors
upward – if properly installed, the MICA instrument connectors should not easily disconnect
from the cluster generator.
4. Remove the flow cell kit base from the workspace area by sliding the base towards the user until the kit
base is no longer seated on the instrument. Users can either retain the flow cell kit lid and base or
dispose in appropriate waste streams.
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