3. Optical measuring principles ABL800 FLEX Reference Manual
Optical system
Measured
parameters
The optical system of the ABL800 FLEX analyzer is designed to measure the
following parameters:
Parameter Description
ctHb concentration of total hemoglobin
sO
2
oxygen saturation
FO
2
Hb fraction of oxyhemoglobin
FCOHb fraction of carboxyhemoglobin
FHHb fraction of deoxyhemoglobin
FMetHb fraction of methemoglobin
FHbF fraction of fetal hemoglobin
ctBil concentration of total bilirubin (the sum of unconjugated
and conjugated bilirubin) in plasma
NOTE: ctBil can be measured on a whole blood or plasma sample. Plasma
samples provide the optimal measurement performance. To obtain optimal
accuracy when following a patient trend in c
tBil, use the same aspiration mode
and the same analyzer.
Hematocrit (Hct) is also available as a derived parameter.
Construction
The optical system is based on a 128-wavelength spectrophotometer with a
measuring range of 478 - 672 nm. The spectrophotometer is connected via an
optical fiber to a combined hemolyzer and measuring chamber.
Sample in
Concave grating
Photodiode array
Slit
Optical fiber
Hemolyze