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Riele 5010 V5+ - Fundamental to the Methods with Standard; Fundamental to the Methods with Multi-Standards; Fundamental to Bichromatic Measurements

Riele 5010 V5+
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5010e_513.doc / 22.01.10
5.1.4 Fundamental to the methods with standard ... 5.1.4 Fundamental to the methods with standard ...
Each measuring of a standard (calibrator) can be executed as single, double or triple determination.
Following is shown:
Each measuring of a standard (calibrator) can be executed as single, double or triple determination.
Following is shown:
In the white reading window the
averaged absorbance of the standard
is shown.
Below the white reading window the
absorbance 1, 2 and 3 of a standard
are shown.
By [OK] the average of all values is
taken over. Values with 0 are ignored
and excluded from the calculation.
The resulting factor is calculated from
the average of the standard.
By [CURS.] a value is selected. A
flashing white square marks the
current value.
By [DEL.] a value is deleted and
excluded from the calculation.
By [RESULT] a measuring is
triggered.
MEASURE ST Glucose
ST[A]
0.616
0.612
0.617
OK CURS. DEL. RESULT
0.615
37.05C 09/08/09 11:39
The determined resulting factor of a standard measurement is stored together with the corresponding
method number. After renewed selection of this method the last resulting factor is offered as "OLD
STD".
The principle of the multiple measurement can also be expanded to all measurements. The
corresponding entry can be set invoking a basic method. The parameter is definable in prepro-
grammed methods (chapter 6 - METHOD EDITOR).
5.1.5 Fundamental to the methods with multi-standards ...
Linear calibration is used in the case of two calibrators. The absorbance forms a linear diagram with
the concentrations (chapter 7.2.2 - Multi-standard functions).
Nonlinear calibration is used for samples with a nonlinear but reproducible connection between the
absorbance and the concentrations. At least three (maximum 20) calibrators are required for nonlinear
calibration (chapter 7.2.2 - Multi-standard functions).
5.1.6 Fundamental to bichromatic measurements …
The calculation procedures based on endpoint measurement (CP 1 to CP 8, CP 13 and CP 14) can be
executed bichromatic. The zero measurement will be done with a wavelength defined as bichromatic.
The bichromatic wavelength might be not included in the standard set of filters. The bichromatic
wavelength can be set after calling a method (chapter 6 METHOD EDITOR Fig. 6.5).
ROBERT RIELE GmbH & Co KG Page 18 5 - CALCULATION PROCEDURES CALCULATION PROCEDURES

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