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General| Pressure Range | Up to 15, 000 psi (1000 bar) |
|---|---|
| Brand | Thermo Scientific |
| Model | UltiMate 3000 |
| Detection Options | UV-Vis, Fluorescence, Refractive Index, Mass Spectrometry |
| Category | HPLC System |
| Pump Type | Binary |
| UV Detection Wavelength Range | 190 to 800 nm |
| Autosampler Temperature Range | 4 - 40 °C |
| Column Oven Temperature Range | 5 - 85 °C |
| Injection Volume | 0.1 - 100 μL |
| Compatibility | Chromeleon |
Explains the meaning of Danger, Warning, and Caution symbols used in manuals and on instruments.
Outlines essential precautions to follow for safe operation and to prevent equipment damage.
Explains the necessity of Operational and Performance Qualification for analytical instrumentation and quality management.
Details OQ/PQ test procedures and their corresponding acceptance limits for OQ and PQ.
Details the procedure for testing UV detector lamp intensity for sensitivity and baseline stability.
Explains measuring wavelength accuracy using a holmium filter in the UV detector.
Describes the importance of UV flow cell transmittance for sensitivity and baseline stability.
Details the procedure for testing UV detector baseline noise and drift.
Explains measuring detector linearity by injecting caffeine standards and analyzing peak height.
Describes the test for autosampler injection volume reproducibility via multiple standard injections.
Explains measuring injection linearity by injecting varying amounts of a caffeine standard.
Details the procedure for testing gradient accuracy by performing a step gradient.
Provides a table of common OQ/PQ test problems, their causes, and recommended solutions.
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