Thermo Scientific UltiMate - Stop Flow

CHROMELEON Listings
OQ/PQ Operating Instructions M1393R0 Page 59 of 60
6.9 Stop Flow
Program:
Wait Sampler.Ready
;Program file to stop the flow and lamp at the end of experiments
;Disable the "stop" sample when further experiments have to be carried out
;System must be setup for Nano LC experiments
;Program version 08/10/2004
;Settings for restrictor (320cm, 15um ID)
Diameter = 75um
Length = 15cm
StationaryPhase = C18_3um_100A
CalibrateCRP When=BeforeFirstSample
;CRP = 625
;ParkPercentage = Disabled
;Pump limits settings
MasterPressure.LowerLimit = 0.0
MasterPressure.UpperLimit = 400.0
ColumnPressure.LowerLimit = 0.0
ColumnPressure.UpperLimit = 200.0
TrapColumnPressure.LowerLimit = 0.0
TrapColumnPressure.UpperLimit = 200.0
;Settings for UV detector
Data_Collection_Rate = 2
UV_VIS_1.Wavelength = 254
TimeConstant = 2
;FAMOS settings
InjectMode = Fullloop
LowDispersionMode = Off
UseAirSegment = Off
UseHeadSpace = Off
RinseBetweenReinjections = Yes
SyringeSpeed = Low
SyringeSpeedFactor = 0.1
SampleHeight = 4
FlushVolume = 5.0
WashVolume = 50
;Commands for FAMOS with cooling option
;Sampler.TempCtrl = On
;Sampler.Temperature.Nominal = 20.00
;Sampler.Temperature.LowerLimit = 5.00
;Sampler.Temperature.UpperLimit = 30.00
;Virtual channel settings
pump_pressure.Formula Formula=masterpressure.value
pump_pressure.Type = Analog
column_pressure.Formula Formula=pump.columnpressure.value
column_pressure.Type = Analog
;loading pump settings
TrapColumnPressure.LowerLimit = 0.0
TrapColumnPressure.UpperLimit = 400.0
;Ultimate pump settings