Thermo Scientific UltiMate - Autosampler Injection Linearity (NAN Configuration)

CHROMELEON Listings
OQ/PQ Operating Instructions M1393R0 Page 53 of 60
6.6 Autosampler Injection Linearity (NAN Configuration)
Sequence: XQ_5_Autosampler Linearity_NAN
Wait Sampler.Ready
;Program file for injection linearity test
;System must be setup for Nano LC experiments
;For this test time constant is 0.5 instead of 2.0
;Program version 08/10/2004
;Settings for restrictor (320cm, 15um ID)
Diameter = 75um
Length = 15cm
StationaryPhase = C18_3um_100A
;CRP = 625
CalibrateCRP When=BeforeFirstSample
;ParkPercentage = Disabled
;Pump limits settings
MasterPressure.LowerLimit = 0.0
MasterPressure.UpperLimit = 400.0
Pump.columnPressure.LowerLimit = 0.0
Pump.columnPressure.UpperLimit = 200.0
TrapColumnPressure.LowerLimit = 0.0
TrapColumnPressure.UpperLimit = 200.0
;Settings for UV detector
Data_Collection_Rate = 2
UV_VIS_1.Wavelength = 272
TimeConstant = 0.5
;FAMOS settings
Wait Sampler.Ready
InjectMode = Partial
LowDispersionMode = Off
UseAirSegment = Off
UseHeadSpace = Off
RinseBetweenReinjections = Yes
SyringeSpeed = Low
SyringeSpeedFactor = 0.1
SampleHeight = 9
FlushVolume = 5.0
WashVolume = 50
;Commands for FAMOS with cooling option
;Sampler.TempCtrl = On
;Sampler.Temperature.Nominal = 20.00
;Sampler.Temperature.LowerLimit = 5.00
;Sampler.Temperature.UpperLimit = 30.00
;Virtual channel settings
pump_pressure.Formula Formula=masterpressure.value
pump_pressure.Type = Analog
column_pressure.Formula Formula=pump.columnpressure.value
column_pressure.Type = Analog
;Ultimate pump settings
Pump.%A.Equate = "100% water + 0.1% FA"
Pump.%B.Equate = "Mob. phase A + 0.8% acetone"