Test Procedures
Page 26 of 60 M1393R0 OQ/PQ Operating Instructions
4.4 Flow Cell Check
The transmittance of the UV flow cell is important for proper detection sensitivity and
baseline stability. The transmittance of the flow cell is read from the SIGNAL screen of the
Setup menu of the UV detector (FIGURE 4-3).
FIGURE 4-3 UV Detector SIGNAL Screen
4.4.1 Performing the Intensity Test of the Flow Cell
To test the flow cell:
a) Make certain that the flow cell to be tested is clean and flushed properly with mobile
phase A.
b) Set the wavelength to 240 nm.
c) Access the SIGNAL screen on the SETUP menu.
d) Monitor the Reference signal (‘ref’) and the Sample signal (‘sig’).
e) Compare the result with the signal intensity limits presented TABLE 2-3 and fill in the
appropriate field in the ‘Other Tests’ page of the QO/PQ report.
SIGNAL: sig: 0.1795
♦ absorbtion ref: 0.3429
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