Thermo Scientific UltiMate - Gradient Accuracy Test (NAN Configuration)

CHROMELEON Listings
OQ/PQ Operating Instructions M1393R0 Page 55 of 60
6.7 Gradient Accuracy Test (NAN Configuration)
Sequence: XQ_6_Gradient Formation Test_NAN
Wait Sampler.Ready
;Program file for gradient formation test
;System must be setup for Nano LC experiments
;The time constant must be set to 0.5 instead of 2.0
;Program version 08/10/2004
;Settings for restrictor (320cm, 15um ID)
Diameter = 75um
Length = 15cm
StationaryPhase = C18_3um_100A
;CRP = 625
CalibrateCRP When=BeforeFirstSample
;ParkPercentage = Disabled
;Pump limits settings
MasterPressure.LowerLimit = 0.0
MasterPressure.UpperLimit = 400.0
ColumnPressure.LowerLimit = 0.0
ColumnPressure.UpperLimit = 200.0
TrapColumnPressure.LowerLimit = 0.0
TrapColumnPressure.UpperLimit = 200.0
;Settings for UV detector
Data_Collection_Rate = 2
UV_VIS_1.Wavelength = 254
TimeConstant = 0.5
;FAMOS settings
InjectMode = Fullloop
LowDispersionMode = Off
UseAirSegment = Off
UseHeadSpace = Off
RinseBetweenReinjections = Yes
SyringeSpeed = Low
SyringeSpeedFactor = 0.1
SampleHeight = 4
FlushVolume = 5.0
WashVolume = 50
;Commands for FAMOS with cooling option
;Sampler.TempCtrl = On
;Sampler.Temperature.Nominal = 20.00
;Sampler.Temperature.LowerLimit = 5.00
;Sampler.Temperature.UpperLimit = 30.00
;Virtual channel settings
pump_pressure.Formula Formula=masterpressure.value
pump_pressure.Type = Analog
column_pressure.Formula Formula=pump.columnpressure.value
column_pressure.Type = Analog
;Ultimate pump settings
pump.%A.Equate = "100% water + 0.1% FA"
%B.Equate = "Mob. phase A + 0.8% acetone"
%C.Equate = "%C"