3-44 Troubleshooting, Testing, and Maintenance
2. Fill reservoirs C and D with solvent 2 (100% methanol with UV
absorbing sample added).
The concentration of UV absorbing sample used in solvent 2 should produce
about 0.45 AUFS in this test.
Flushing solvent lines
Be sure to flush any residual solvents from the system.
Adjusting the recorder
To adjust the recorder:
1. Set the detector to 0.5 AUFS.
2. Pump solvent 1 (reservoir A or B) isocratically at 5 mL/min and adjust
the recorder
to 0.
3. Pump solvent 2 (reservoir C or D) isocratically at 5 mL/min, and adjust
the recorder to full scale using the recorder’s variable full scale input.
The recorder is now set to respond so that 0% composition (pure methanol in
solvent 1) = 0% full scale, and 100% composition (methanol containing sample
in solvent 2) = 100% full scale.
Program the gradient table
Enter and save Gradient table values as shown below.
Gradient table values
Time Flow %A %B %C %D Curve
Initial1.00100000*
5.00 1.00 90 0 10 0 11
10.001.0010000011
15.00 1.00 90 0 0 10 11
20.00 1.00 0 100 0 0 11
25.00 1.00 0 90 10 0 11
30.00 1.00 0 100 0 0 11
35.00 1.00 0 90 0 10 11
40.00 1.00 0 100 0 0 11
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