Waters Xevo TQ-S cronos - MS;MS Operating Modes

Table 1–2: MS operating modes: (continued)
Operating mode MS1 Collision cell MS2
SIR Pass all masses Resolving (static)
In MS mode, the instrument can acquire data at scan speeds as high as 20,000 Da/s. Use this
mode for instrument tuning and calibration before MS/MS analysis. See the mass spectrometer’s
online Help for further information.
Use the selected ion recording (SIR) mode for quantitation when you cannot find a suitable
fragment ion to perform a more specific multiple reaction monitoring (MRM) analysis (see MRM
mode (Page 34) for further details). In SIR and MRM modes, neither quadrupole is scanned,
and no spectrum (intensity versus mass) is produced. The data obtained from SIR or MRM
analyses derive from the chromatogram plot—specified mass intensity (SIR) or specified
transition (MRM) versus time.
1.7 MS/MS operating modes
The following table shows the MS/MS operating modes.
Table 1–3: MS/MS operating modes:
Operating mode MS1 Collision cell MS2
Product (daughter) ion
spectrum
Static (at precursor
mass)
Fragment precursor
ions and pass all
masses
Scanning
Precursor (parent) ion
spectrum
Scanning Static (at product
mass)
MRM Static (at precursor
mass)
Static (at product
mass)
PICS (Product Ion
Confirmation Scan)
Static (at precursor
mass)
Switching between
static (at product
mass) and scanning
Constant neutral loss
spectrum
Scanning
(synchronized with
MS2)
Scanning
(synchronized with
MS1)
RADAR is an additional mode that allows you to create experiments containing both MS and
MS/MS functions.
January 10, 2022, 715006200 Ver. 01 (previously released as Rev. A)
Page 32