48 World Precision Instruments
APPENDIX E: FREQUENTLY ASKED QUESTIONS
1. When do researchers want to use the blank function?
Answer: The blank function allows researchers to subtract out resistance
measurement that is not caused by the cell layer. This could be a reading aected
by the electrodes or uid resistances or other factors. Normally, you would
measure a blank insert with the same volumes as the insert and keep subtracting
that blank value from the samples. This way, any oset contributed by the
electrode is nullied. The subtracted value is the resistance contributed by the
cellular layer.
3. Does the system automatically calculate TEER?
Answer: No. Calculating TEER requires an area calculation. See "TEER Calculations"
on page 2 and "Appendix D: Resistance Calculations" on page 46.)
4. The program is automatically stored when the last well is reached. What happens
when I only want to measure 8 of 96 wells and store the program?
Answer: Once you’re done measuring, open the settings, press the store screen
menu, then store the plate data to the USB.
To Next Page
Loading...
