WPI EVOM - TEER Calculations

2 World Precision Instruments
Repeat the sampling process. Press the Preview button to see the measurements
that have been taken.
6. Once the last well has been recorded, the le storing selection notication appears
prompting you to take an action. Select one of the options as follows:
• Export starts the storage of all the plate les. When you select it a message
appears describing which plate is being stored. The number of plates
could be one, two or three, depending on how many were collected before
exporting.
• Next returns you to the main screen so you can take measurements of the
next plate which starts from a clear state.
NOTE: If you are on plate 3, the Next button does not appear, because EVOM™
Manual’s memory only holds data from three plates.
• Back returns you to the main screen (data collection). You go back to the rst
well of the current plate being collected and can acquire the data again.
7. When the plate data has been saved, a conrmation message appears. Press the
Done button to return to the main screen.
8. Now you can work with your data.
• If you stored your data on the USB ash drive, remove the drive and transfer
the data les to your computer. They are in comma separated value (CSV) le
format for Microsoft
®
Excel.
• If you stored your data using the EVOM™ Companion Application, all your
data les will be located in the directory you specied when you started the
Companion Application.
TEER Calculations
To compute TEER, multiply the measured resistance by the surface area’s listed below.
For example, a 12 mm insert measures 565 Ω, the TEER is 565Ω * 1.13 or 638 Ω-cm
2
.
• 6 well plate (24 mm inserts) 4.52 cm
2
• 12 well plate (12 mm inserts) 1.13 cm
2
• 24 well plate 6.5 mm inserts) 0.3316 cm
2
• 96 well plate (4.3 mm inserts) 0.145 cm
2