OPERATION
Axio Observer Illumination and contrast techniques ZEISS
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Key to Fig. 148:
1 Standby button
2 Left sideport
3 Coarse / fine focus drive (left side) with finger wheel for fine focus, flat
4 Vertical stop for focus drive
5 Light path selector wheel (left / right sideport / vis)
6 Light path selector wheel (baseport / vis / frontport)
7 Microscope stage with inserted universal mounting frame K
8 Condenser centering screws
9 Polarizer D with 2-position filter changer or 3-position filter changer
10 Luminous-field diaphragm knurled wheel
11 LCD display
12 HAL 100 illuminator
13 Vertical adjustment knob for condenser
14 Carrier for transmitted-light illumination
15 Condenser (manual or motorized)
16 Slot F for iris diaphragm slider as reflected light luminous-field diaphragm (manual or motorized)
17 Slot A for iris diaphragm slider as reflected light aperture diaphragm (manual or motorized) or FL attenuator
(manual or motorized)
18 Slot for 3-position filter slider, d=25 mm
19 Colibri 7 illumination system
20 LM-Set button
21 Drive knobs for controlling XY positioning of the mechanical stage
22 Right sideport
23 6-position nosepiece H DIC M27 coded
24 Slot for 3-position contrast slider 10x29 mm for PlasDIC module and analyzer
25 Slot for DIC / PlasDIC slider
26 Reflector turret (coded or motorized)
27 Optovar turret selector wheel (max. 3 positions)
28 Coarse / fine focus drive (right side)
29 Control ring, right
30 TL button for switching the transmitted light LED / halogen illuminator on and off or for opening and closing the
transmitted light shutter
31 RL button for switching the reflected light shutter (fluorescence) on and off
32 Control wheel for LED light / halogen illuminator intensity control
33 Frontport
34 Binocular tube
35 Binocular section of the tube
36 Eyepiece
37 Eyepiece focusing ring
5.12.1.1 General operating principle
Transmitted light bright field microscopy is the simplest of all optical microscopy techniques, since it
allows high contrast and stained specimens (e.g. blood smears) to be viewed quickly and easily.
In addition to so-called direct beam bundles, indirect bundles which are diffracted and scattered by the
specimen details, are of major importance for obtaining as true an image as possible. The greater the
portion of indirect bundles (aperture), the truer, according to ABBE, the microscope image.
To make use of the microscope's full optical performance, and in particular that of the objective, the
condenser, luminous-field diaphragm and aperture diaphragm should be adjusted in line with the
requirements for KÖHLER illumination. This basic rule of microscopy is described in detail in
section 5.12.1.3.
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