OPERATION
Axio Observer Illumination and contrast techniques ZEISS
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(4) Reflected light C-DIC
• Prepare the microscope for reflected light brightfield.
• Rotate reflector turret to swivel C-DIC/TIC P&C reflector module (Fig. 154/7) into the beam path.
• Insert the C-DIC slider 6x20 (Fig. 158/1) into the slot for C-DIC slider.
• If necessary, refocus and rotate the selector wheel on the C-DIC slider 6x20 (Fig. 158/1) until the
structure of interest is visible at maximum contrast.
• Additional contrasting effects can be produced by adjusting the selector wheel on the C-DIC slider.
(5) Additional note
Differential interference contrast or differential interference contrast with circularly polarized light is
created by a (pseudo) relief in the specimen. Therefore, it depends on whether linear structures are
oriented in the "light-shadow" direction (very faint contrast) or vertical to this direction (maximum
contrast).
If you use the C-DIC slider 6x20, you can turn the selector wheel of the C-DIC slider to align the
structures vertically to the "light-shadow" direction and thus achieve maximum contrast.
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