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Click No to create new regions that are not “connected”. The region will be inserted in
the dot plots of all other samples. When the properties of the region are changed, the
changes affect only the selected sample.
The region is copied to all samples of the assay.
How to Work with Gates in Dot Plots
You can insert gates only in generic assays. For predefined assays, the gate is already
defined.
Before you can insert a gate, you have to draw a region (see “How to Add Regions to Dot
Plots (Generic Assay only)” on page 234). If a gate is already set, you first have to remove
the existing gate.
To add a gate to a region:
1 Left-click the region border to select the region to which you want to add the gate.
The gating buttons in the toolbar are enabled.
2 Click the Horizontal Gate button or the Vertical Gate button to set a gate along
the horizontal or the vertical borders of the selected region.
In the result table, a value appears in the % of gated column.
If the gating direction is already set, you first have to remove the existing gate:
1 Left-click the region border to select the region.
If a gate already exists, the Remove Gate button is enabled.
2 Click this button.
The gate is removed and the gating buttons are enabled.
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