Agilent InfinityLab LC Series 1260 Infinity II Isocratic- and Quaternary Pumps User Manual 59
Using the Pump
3
Hints for Successful Use of the Pump
Hints for Successful Use of the Pump
• Always place the solvent cabinet with the solvent bottles on top of the
quaternary pump (or at a higher level).
• When using salt solutions and organic solvents in the quaternary pump it is
recommended to connect the salt solution to one of the bottom gradient
valve ports and the organic solvent to one of the upper gradient valve ports.
It is best to have the organic channel directly above the salt solution
channel. Regular flushing of all MCGV channels with water is recommended
to remove all possible salt deposits in the valve ports.
• Before operating the quaternary pump, flush the pump and vacuum
degasser. This is especially recommended if it has been turned off for some
time (for example, overnight) and volatile solvent mixtures are used in the
channels.
• Prevent blocking of solvent inlet filters. Never use the pump without solvent
inlet filter. Prevent the growth of algae, see “Prevent Blocking of Solvent
Filters” on page 82).
• Regularly check the purge valve frit and column frit. A blocked purge valve
frit can be identified by a black or yellow surface, deposits or by a pressure
greater than 10 bar, when pumping distilled water at a rate of 5 mL/min
with an open purge valve.
• When using the quaternary pump at low flow rates (for example,
0.2 mL/min) check all 1/16-inch fittings for any signs of leaks.
• Whenever exchanging the pump seals the purge valve frit should be
exchanged, too.
• When using buffers or other salt solutions, flush the system with water
before switching it off. The seal wash option should be used when salt
concentrations of 0.1 M or higher will be used for long time periods.
• Check the pump pistons for scratches when changing the piston seals.
Scratched pistons will cause micro leaks and will decrease the lifetime of
the seal.
• Pressurize the system according to the wear in procedure after changing
the piston seals (see “Maintenance of a Pump Head Without Seal Wash
Option” on page 155).
• Consider recommendations given in the solvent information section, see
“Solvent Information” on page 71.
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