Agilent InfinityLab LC Series 1260 Infinity II Isocratic- and Quaternary Pumps User Manual 89
Optimizing Performance
4
When to use the Seal Wash Function
When to use the Seal Wash Function
Highly concentrated buffer solutions will reduce the lifetime of the seals and
pistons in your pump. The seal wash function allows to maintain the seal
lifetime by flushing the back side of the seal with a wash solvent.
The seal wash function is strongly recommended when buffer concentrations
of 0.1 M or higher will be used for long time periods in the pump.
The seal wash function comprises a support ring, secondary seal, gasket and
seal holder for both piston sides. Place a wash bottle filled with 90 % water /
10 % isopropanol above the pump in the solvent cabinet and guide the SW
waste into the waste bottle underneath the LC stack. The peristaltic pump
moves a flow through the pump head removing all possible buffer crystals
from the back of the pump seal. This mixture prevents growth of algae or
bacteria in the wash bottle and reduces the surface tension of the water.
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