84 Agilent InfinityLab LC Series 1260 Infinity II Isocratic- and Quaternary Pumps User Manual
3
Using the Pump
Normal Phase Applications
Seals for Normal Phase Applications
For running normal phase applications on 1200 Infinity Series pumps, yellow
PE seals are required, which exist as piston seals and wash seals. Seal wash is
very uncommon for normal phase applications (no buffers needed), but wash
seals are needed for seal wash pump heads.
1290 Infinity pumps use PE seals by default. In combination with ceramic
pistons, PE seals are used for both reversed phase (1200 bar) and normal
phase applications.
1260 Infinity pumps use sapphire pistons and black PTFE piston and wash
seals by default (600 bar). Such PTFE seals create small wear particles in
normal phase applications, which can clog valves and other parts in the flow
path.
PE seals have a limited life time when used with normal phase solvents and
sapphire pistons. Agilent recommends a maximum pressure of 200 bar for this
combination, which shall also be applied for pressure tests.
Choice of Normal Phase Valves and Seals
Table 1 7 Recommended valves and seals for normal phase applications
1260 Infinity 1290 Infinity
Inlet valves 1260 Infinity Inlet Valve Type N
(G1312-60166)
1290 Infinity Inlet Valve Type N
(G4220-60122)
1290 Infinity Quat Inlet Valve
Type N (G4204-60122)
Outlet valves 1260 Infinity Outlet Valve Type
N/SFC (G1312-60167)
1290 Infinity Outlet Valve Type
N (G4220-60128)
Seals PE seals (pack of 2)
(0905-1420)
Wash Seal PE (0905-1718)