Agilent InfinityLab LC Series 1260 Infinity II FLD User Manual 103
Optimizing the Detector
5
Method Development
The two upper traces
are obtained using
two different
excitation
wavelengths.
The lower trace is a
pure standard of the
known impurities.
Figure 35 Qualitive analysis of MBC (2-benzimidazole carbamic acid methylester) and
impurities
DPLQR2+SKHQD]LQH
GLDPLQRSKHQD]LQH
6WDQGDUG
7LPH>PLQ@
8QNQRZQ
Table 11 Conditions for analysis of DAP and MBC according to figures above
Column Zorbax SB, 2 x 50 mm, PNA, 5 µm
Mobile phase A = water; B = acetonitrile
Gradient 0 minutes, 5%
10 minutes, 15%
Flow rate 0.4 ml/min
Column temperature 35 °C
Injection volume 5 µl
FLD settings PMT 12,
response time 4 s,
step size 5 nm
Ex 265nm and 430nm
Em 540 nm
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