Agilent InfinityLab LC Series 1260 Infinity II FLD User Manual 89
Optimizing the Detector
5
Getting Started and Checkout
Setting the Chromatographic Conditions
1 Set up the system with the following chromatographic conditions and wait
until the baseline gets stable.
Table 6 Chromatographic Conditions
Mobile phases A = water = 35 %
B = Acetonitrile = 65 %
Column OSD-Hypersil column, 125 mm x 4 mm i.d. with 5
µm particles
Sample Isocratic standard sample, 1:10 diluted in
methanol
Flow rate 1.5 ml/min
Compressibility A (water) 46
Compressibility B (Acetonitrile) 115
Stroke A and B auto
Stop time 4 min
Injection volume 5 µl
Oven temperature (1200) 30 °C
FLD Excitations/Emission Wavelength EX = 246 nm, EM = 317 nm
FLD PMT Gain PMT = 10
FLD Response time 4 s
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