218 Agilent InfinityLab LC Series 1260 Infinity II FLD User Manual
9
Maintenance
Flow Cell Flushing
Flow Cell Flushing
1 Flush with bidistilled water.
2 Flush with nitric acid (65 %) using a glass syringe.
3 Leave this solution in the cell for about one hour.
4 Flush thoroughly with bidistilled water at a higher flow rate, e.g.
1.5 mL/min.
When If flow cell is contaminated
Tools required Description
Glass syringe
Adapter
Parts required # Description
1 Bidistilled water, nitric acid (65 %), tubings to waste
Dangerous concentration of nitric acid
The nitric acid flushing procedure is not an infallible remedy for a dirty cell. It is to
be used as a last attempt to salvage the cell before cell replacement. Note that the
cell is a consumable item.
➔ Give proper attention to safety.
Aqueous solvents in the flow cell can built up algae. Algae do fluoresce. Therefore do not
leave aqueous solvents in the flow cell for longer periods. Add a small percentage of
organic solvents (e.g. Acetonitrile or Methanol ~5 %).
Do not exceed the pressure limit of 20 bar (0.2 MPa).
Do not use the HPLC pump to pump nitric acid through the system.
To Next Page
Loading...
Need help?
General
