Agilent InfinityLab LC Series 1260 Infinity II FLD User Manual 105
Optimizing the Detector
5
Method Development
Table 12 Conditions for simultanoeus multi wavelength detection for PNA-analysis (see
figure below)
Column Vydac, 2.1 x 250 mm, PNA, 5 µm
Mobile phase A = water; B = acetonitrile (50 : 50)
Gradient 3 min, 60 %
14.5 min, 90 %
22.5 min, 95 %
Flow rate 0.4 mL/min
Column temperature 22 °C
Injection volume 2 µL
FLD settings PMT 12,
response time 4 s
The upper trace was
received with
traditional
wavelength
switching.
Figure 36 Simultaneous multi wavelength detection for PNA-analysis
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