70 Agilent InfinityLab LC Series 1260 Infinity II FLD User Manual
3
Using the Fluorescence Detector
Special Settings
Fluorescence Scan Range (G7121B ONLY)
The Fluorescence Scan Range panel enables you to set up
a fluorescence scan (3D scan). The fluorescence scan can
be used to collect emission and excitation spectral
information in a single task. It is most suitable for the
characterization of single compounds, and for checking the
purity of eluents. The contents of the flow cell must be kept
constant during the measurement; use off-line
measurements with the FLD cuvette or stopped-flow
conditions in chromatography.
Enter the wavelength ranges for excitation and emission.
The Step size determines the number of data points per
spectrum. The time per scan (in seconds) is calculated
automatically and displayed.
The Lamp section allows you to specify how the lamp is
used in an analysis.
Select Only On During Run to specify that the lamp is
switched on only during the run time, and is switched off
when the run finishes.
Select Economy Mode to run the lamp in a low frequency,
low current mode. This increases the lamp lifetime at the
expense of sensitivity.
To prolong the life of the lamp, clear the check box if you
have a multiple detector configuration and do not want to
use the FLD for the next analyses.
NOTE: If the Lamp on required for analysis check box is
marked, but the lamp is off when the run is started, the
lamp is switched on automatically.
Lamp Energy Reference
Choose On to switch on a compensation for flash-to-flash
variations in lamp intensity using the reference diode. This
gives improved signal-to-noise.
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