Andor Technology iXon3 Series - Photon Counting by Post-Processing

Page 61
iXon 3
Features & Functionality
3.10.1 - Photon Counting by Post-Processing
Asapost-processinganalysis,theuserholdstheexibilityto‘trialanderror’photoncountapre-recordedkinetic
series, trading-off temporal resolution vs SNR by choosing how many images should contribute to each photon counted
accumulated image.
For example, a series of 1000 images could be broken down into groups of 20 photon counted images, yielding 50 time
points. If it transpires that better SNR is required, the original dataset could be re-treated using groups of 50 photon
counted images, yielding 20 time points.
Figure 43: Schematic illustration of how photon counting can be applied to a kinetic series as a post processing step, affording increased
exibility in ‘trail and error’ trading SNR vs temporal resolution.