Applied Biosystems 7500 Fast User Manual

Chapter 5 Performing the Background Calibration and Optical Calibration
Performing the Optical Calibration for the 7500/7500 Fast System
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Installation and Maintenance Guide 63
Notes
5
Analyzing the Optical Calibration Data
1. In the SDS software, click (or select AnalysisExtract).
The software extracts the optical calibration, then displays a message indicating the
extraction is complete.
2. Click .
3. In the SDS software, select FileClose.
Unloading the
Plate
PHYSICAL INJURY HAZARD. During instrument operation, the
sample block can be heated to 100 °C. Before performing the following procedure, be
sure to wait until the sample block reaches room temperature.
1. Remove the ROI calibration plate:
a. Press the tray to open it.
b. Remove the ROI calibration plate.
c. Press the tray to move it into the instrument.
2. Place the ROI calibration
plate inside its packaging
sleeve, then return it to the
spectral calibration kit in
the freezer.
IMPORTANT! Do not discard the ROI calibration plate. If you store the plate in its
original packaging sleeve, you can use the plate up to three times after you open it.
Continue with “Performing the Pure Dye Calibration” on page 67.
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Applied Biosystems 7500 Fast Specifications

General

Brand	Applied Biosystems
Model	7500 Fast
Category	Laboratory Equipment
Language	English